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The curriculum covers outpatient and pro-fee 1500 documentation across a full range of service types: E/M office visits, operative notes, inpatient and observation services, emergency department, discharge services, and consultations

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The cells were incubated overnight at 37 °C on Luria-Bertani-ampicillin agar plates and transformants were selected and grown overnight in 50 mL of LB medium containing 100 μg/mL of ampicillin at 37 °C